Allied BioNutrition’s Nutraceutical biotechnology focuses on the following nutritional and medical areas, utilizing a clinically-based, field-proven, proprietary Licorice Root Extract manufactured in Israel:
- Arterial health
- Cholesterol management
- Blood pressure management
Note: This biotechnology science will be used to develop better supplements for optimal heart health, and in approximately one year, a new line of medical foods for the dietary management of heart disease.
In April 2016, the results from our double-blind, placebo-controlled study were published in the scientific journal Food and Nutrition Research. The study examined the effect of our unique Licorice Root Extract on the thickness of the artery wall (carotid intima-media thickness or CIMT) in individuals with oxidation in the arteries. CIMT is considered a strong biomarker of overall cardiovascular and arterial health.
People with increased oxidation in the arteries, total cholesterol greater then 240mg/dL, were randomly allocated to 2 groups as follows: an experimental group that received 200mg of the licorice root extract and a placebo group. 110 participants were initially allocated to the 2 groups. After some withdrawals, 94 subjects ultimately completed the trial. After 1 year, the study yielded the following results:
- In the experimental group the thickness of artery (CIMT) decreased from 0.92 mm to 0.84 mm. In the placebo group CIMT increased from 0.85 to 0.88.
- In the experimental group total cholesterol decreased from 284 to 262 mm/dL. In the placebo group total cholesterol decreased from 291 to 289mm/dL.
- In the experimental group the LDL (bad) cholesterol decreased from 183 to 173 mm/dL. In the placebo group the LDL (bad) cholesterol increased from 177 to 179.
- HDL or good cholesterol did not change in either group.
- In the experimental group systolic blood pressure decreased from 138 to 125mmhg. In the placebo group systolic blood pressure increased from 136 to 137.
- In the experimental group diastolic blood pressure decreased from 92 to 84. In the placebo group diastolic blood pressure increased from 89 to 90.
These results were extremely compelling. Following one year of licorice root extract consumption, mean CIMT, total cholesterol, LDL levels, and blood pressure decreased. This suggests that this ingredient may attenuate the development of oxidation and of related cardiovascular problems.
In 2016, Allied BioNutrition will launch a family of Nutraceutical products that contain our patented, proprietary Licorice Root Extract. Currently, in Israel, Allied BioNutrition initiated distribution in this pilot market, where thousands of consumers are already using our proprietary Licorice Root Extract daily, and are realizing its benefits. In 2016, Allied BioNutrition will launch a family of Nutraceutical products that contain this patented, proprietary Licorice Root Extract, bringing this extraordinary bio-technology, with all its unique cardiovascular health benefits, to the world.
The Licorice Root
Licorice Root, Glycyrrhiza glabra, has a long history of use by cultures throughout the world, including the Greeks, Egyptians, Chinese and Hindus. It grows wild in southern and central Europe and is extensively cultivated in Russia, Spain, Persia and India. Licorice is widely used as a flavoring, not only for candy, but also in cough drops, syrups, tonics and laxatives.
The root of Glycyrrhiza glabra has been used for centuries as antidotes, demulcents, expectorants, and remedies for allergic inflammation, as well as flavoring and sweetening agents in Asia and Europe(1). Licorice contains glycyrrhizin, oleane triterpenoids, glucose, and flavonoids. It has been used in folk medicine for addressing various disorders. Licorice has the biological capabilities of detoxification, antioxidation, and anti-infection.
Oxidative modification of low-density lipoprotein (LDL) in the arterial wall plays a key role in the pathogenesis of the arterial wall. Under oxidative stress, LDL is exposed to oxidative modifications by arterial wall cells, including macrophages. Oxidative stress also induces cellular-lipid peroxidation, resulting in the formation of ‘oxidized macrophages’, which demonstrate increased capacity to oxidize LDL and increased uptake of oxidized LDL(2). Macrophage-mediated oxidation of LDL depends on the balance between pro-oxidants and antioxidants in the lipoprotein and in the cells. LDL is protected from oxidation by antioxidants. A large number of studies provide data suggesting that consumption of dietary antioxidants is associated with promoting cardiovascular health. Basic research provides plausible mechanisms by which dietary antioxidants might reduce the development of oxidation. These mechanisms include inhibition of LDL oxidation, inhibition of cellular lipid peroxidation and consequently attenuation of cell-mediated oxidation of LDL. The Licorice root includes potent polyphenolic flavonoids.
Interestingly, consumption of some plant-derived flavonoids results in their absorption and appearance in plasma and tissues. The inverse relationship between dietary flavonoids consumption and cardiovascular problems may be associated with the ability of flavonoids to attenuate LDL oxidation, macrophage foam cell formation, and increased oxidation. The effect of flavonoids on arterial cell-mediated oxidation of LDL is determined by their accumulation in the lipoprotein and in arterial cells, such as macrophages. Flavonoids can reduce LDL lipid peroxidation by scavenging free radicals, chelation of transition metal ions and sparing of LDL-associated antioxidants.
Glabridin is a polyphenolic flavonoid and a main constituent in the hydrophobic fraction of licorice extract. It is well known for its beneficial effects on the skin through an inhibitory effect on melanogenesis and inflammation(3). It also has a wide range of biological activities, including inhibiting the growth of microorganisms, protecting against oxidation, promoting kidney health, and promoting cardiovascular protective activities(4,5). Recently, it was reported that glabridin has a protective effect on low-density lipoprotein oxidation, and this contributes to the antioxidation effect of glabridin(6,1,11).
Licorice extract has been known to have an anti-inflammatory effect. Various components of licorice extract, including glabridin, are reported to be involved in this effect by exerting an inhibitory effect on cyclooxygenase and lipoxygenase activities(3,9).
According to a 1997 study published in the American Journal of Clinical Nutrition(4), dietary supplementation of each E zero mouse with licorice (200 micrograms/d) or pure glabridin (20 micrograms/d) for six weeks resulted in a substantial reduction in the susceptibility of their LDL to oxidize, along with a reduction in the oxidative lesion area.
In a human study from Lipid Research Laboratory(7), Technion Faculty of Medicine Rambam Medical Center, Haifa, Israel, demonstrated that Licorice consumption (using a proprietary Licorice Root Extract) produced the following results:
- Reduced patients’ plasma susceptibility to oxidation by 19%
- Increased resistance of plasma LDL against three major oxidation biomarkers:
- LDL oxidation (by 55%)
- Aggregation (by 28%)
- Retention, estimated as chondroitin sulfate binding ability, by 25%
- Reduced plasma cholesterol levels (by 5%), which was due to a 9% reduction in plasma LDL cholesterol levels
- Reduced by 14% plasma triacylglycerol levels. After the 1 month of placebo consumption, these parameters reversed toward baseline levels. Licorice extract supplementation also reduced systolic blood pressure by 10%.
In two recent random order and double-blind, placebo controlled studies(12,13), 22 subjects consumed the proprietary Licorice Root Extract or placebo for 6 months. The subjects’ oxidative stress level, as well as plasma LDL oxidation, decreased by 20%.
Professor Michael Aviram, Ph.D., Creator, Proprietary Licorice Root Extract
Professor Michael Aviram, Ph.D., is the creator of Allied BioNutrition’s proprietary Licorice Root Extract. He directs the Lipid Research Laboratory. Professor Aviram holds the Bernice R. & Joseph Tanenbaum Chair in Preventive Medicine at the Rappaport Faculty of Medicine, Technion-Israel Institute of Science & Technology, and at Rambam Health Care Campus (where he is the Director of the Clinical Research Institute of Rambam). Professor Aviram is the Professor’s Representative on the Technion Council and on the Technion Board of Governors (BOG). He also acts as the Senior Vice Dean of the Technion Faculty of Medicine. Professor Aviram is a leading expert and opinion leader in cholesterol and heart research, particularly in the role of oxidative stress and antioxidants focusing on dietary polyphenols and paraoxonases. He was the first to elucidate the mechanisms behind the protection against oxidation that is provided by consumption of red wine or pomegranate juice (POM), as well as the cardio-protection of the antioxidant enzyme—paraoxonases (PON)—which breaks down harmful oxidized lipids.
Professor Aviram spearheaded the research and formulation using his proprietary Licorice Root Extract that is the main component of Allied BioNutrition’s Nutraceutical products, using a patented technology that reduces oxidation in the blood by more than 55%. A recent breakthrough study indicates a significant improvement in arterial health. Moreover, biomarkers for blood pressure, cholesterol, and glucose, also improved.
- 1. Belinky PA, Aviram M, Fuhrman B, Rosenblat M, and Vaya J (1998) The antioxi-dative effects of the isoflavanglabridin on endogenous constituents of LDL during its oxidation. Atherosclerosis 137: 49-61.
- 2. Rizzo M,Banach M Et al. Lipid-lowering therapies and achievement of LDL-cholesterol targets.Arch Med Sci. 2012 September 8; 8(4): 598–600.
- 3. Yokota T Et al. The inhibitory effect of glabridin from licorice extracts on melanogenesis and inflammation, Pigment Cell Res. 1998 Dec;11(6):355-61
- 4. Fuhrman B, Buch S, Vaya J, Belinky PA, Coleman R, Hayek T, and Aviram M (1997) Licorice extract and its major polyphenol glabridin protect low-density lipoprotein against lipid peroxidation: in vitro and ex vivo studies in humans and in athero-sclerotic apolipoprotein E-deficient mice. Am J ClinNutr 66: 267-275
- 5. Fukai T, Satoh K, Nomura T, and Sakagami H (2003) Preliminary evaluation of antinephritis and radical scavenging activities of glabridin from Glycyrrhizaglabra. Fitoterapia 74: 624-629
- 6. Vaya J., Belinky P. and Aviram M. Antioxidant constituents from licorice roots: isolation, structure elucidation and antioxidative capacity toward LDL oxidation. Free Radic. BioI Med. 23 :302-313 (1997)
- 7. Fuhrman B., VolkovaN.,Kaplan M., Presser D., Attias J., Hayek T. and Aviram M. Anti-atherosclerotic effects of licorice extract supplementation to hypercholesterolemic patients: increased resistance of their LDL to atherogenic modifications, reduced plasma lipids levels, and decreased systolic blood pressure. Nutrition 18: 268-273 (2002)
- 8. Kent UM et al. The licorice root derived isoflavanglabridin inhibits the activities of human cytochrome P450S 3A4, 2B6, and 2C9. Drug MetabDispos. 2002 Jun;30(6):709-15
- 9. Inoue H, Saito H, Koshihara Y, and Murota S (1986) Inhibitory effect of glycyrrhetinic acid derivatives on lipoxygenase and prostaglandin synthetase. Chem Pharm Bull 34: 897-901
- 10. Kent UM etal.The licorice root derived isoflavanglabridin inhibits the activities of human cytochrome P450S 3A4, 2B6, and 2C9. Drug MetabDispos. 2002 Jun;30(6):709-15
- 11. Fuhrman B. and Aviram M. Flavonoid protect LDL from oxidation and attenuate atherosclerosis. Curr. Opin. Lipidol.12: 41-48 (2001).
- 12. Carmeli E, Harpaz Y, Kogan NN, Fogelman Y. The effect of an endogenous antioxidant glabridin on oxidized LDL. J Basic ClinPhysiolPharmacol. 2008;19(1):49-63.
- 13. Carmeli E, Fogelman Y. Antioxidant effect of polyphenolic glabridin on LDL oxidation. ToxicolInd Health; 2009 May ;25(4-5):321-324
DISCLAIMER: THIS MATERIAL IS FOR CONSUMER INFORMATIONAL PURPOSES ONLY. NOTHING IN THIS WEBSITE IS INTENDED AS, OR SHOULD BE CONSTRUED AS, MEDICAL ADVICE. CONSUMERS SHOULD CONSULT WITH THEIR OWN HEALTHCARE PRACTITIONERS FOR INDIVIDUAL, MEDICAL RECOMMENDATIONS. THE INFORMATION IN THIS WEBSITE CONCERNS A DIETARY SUPPLEMENT, AN OVER-THE-COUNTER PRODUCT THAT IS NOT A DRUG. OUR DIETARY SUPPLEMENT PRODUCTS ARE NOT INTENDED FOR USE AS A MEANS TO CURE, TREAT, PREVENT, DIAGNOSE, OR MITIGATE ANY DISEASE OR OTHER MEDICAL OR ABNORMAL CONDITION.